Function and Biology Details
Reactions catalysed:
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Immunoglobulin C1-set
- MHC class I alpha chain, alpha1 alpha2 domains
- Beta-2-Microglobulin
- Immunoglobulin/major histocompatibility complex, conserved site
- Immunoglobulin-like domain
- Immunoglobulin-like domain superfamily
- Immunoglobulin-like fold
- MHC classes I/II-like antigen recognition protein
2 more domains
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137835 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains:
Structure domains:
Length: 274 amino acids
Theoretical weight: 31.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
P04439 (Residues: 25-298; Coverage: 80%)
Sequence domains:
Structure domains:
Beta-2-microglobulin
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Pol protein
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.01 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
Length: 9 amino acids
Theoretical weight: 1.01 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
- Canonical: Q9YV12 (Residues: 313-321; Coverage: 1%)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
RIGAKU MICROMAX-007
Spacegroup:
P21
Expression systems:
- Escherichia coli
- Not provided
