› 3rle

X-ray diffraction
1.65Å resolution

Crystal Structure of GRASP55 GRASP domain (residues 7-208)

Released:
DOI: 10.2210/pdb3rle/pdb
PDB entry 3rle coloured by chain, front view.
PDB entry 3rle coloured by chain, side view.
PDB entry 3rle coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the membrane-tethering GRASP domain reveals a unique PDZ ligand interaction that mediates Golgi biogenesis.
Truschel ST, Sengupta D, Foote A, Heroux A, Macbeth MR, Linstedt AD
J Biol Chem 286 20125-9 (2011)
PMID: 21515684

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190688 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Golgi reassembly-stacking protein 2 Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 22.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H8Y8 (Residues: 2-208; Coverage: 46%)
Gene names: GOLPH6, GORASP2
Sequence domains: GRASP55/65 PDZ-like domain
Structure domains: Pdz3 Domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P41212
Unit cell:
a: 88.04Å b: 88.04Å c: 61.195Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.222
Expression system: Escherichia coli

Quick links

Citations

11 review citations

GRASP: A Multitasking Tether.
Rabouille et al. (2016)
10 more

10 mentions without citation

Plasticity of PDZ domains in ligand recognition and signaling.
Ivarsson Y. (2012)
9 more