› 3rln

X-ray diffraction
2.25Å resolution

Structural Basis of Cytosolic DNA Recognition by Innate Immune Receptors

Released:
DOI: 10.2210/pdb3rln/pdb
PDB entry 3rln coloured by chain, front view.
PDB entry 3rln coloured by chain, side view.
PDB entry 3rln coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the HIN domain:DNA complexes reveal ligand binding and activation mechanisms of the AIM2 inflammasome and IFI16 receptor.
Jin T, Perry A, Jiang J, Smith P, Curry JA, Unterholzner L, Jiang Z, Horvath G, Rathinam VA, Johnstone RW, Hornung V, Latz E, Bowie AG, Fitzgerald KA, Xiao TS
Immunity 36 561-71 (2012)
PMID: 22483801

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-interferon-inducible protein 16 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16666 (Residues: 571-766; Coverage: 25%)
Gene names: IFI16, IFNGIP1
Sequence domains: HIN-200/IF120x domain
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: R32
Unit cell:
a: 128.673Å b: 128.673Å c: 67.772Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.177 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

179 review citations

Activation and regulation of the inflammasomes.
Latz et al. (2013)
178 more

3 mentions without citation

Immunobiology and structural biology of AIM2 inflammasome.
Wang et al. (2020)
2 more