› 3rnu

X-ray diffraction
2.5Å resolution

Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors

Released:
DOI: 10.2210/pdb3rnu/pdb
PDB entry 3rnu coloured by chain, front view.
PDB entry 3rnu coloured by chain, side view.
PDB entry 3rnu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the HIN domain:DNA complexes reveal ligand binding and activation mechanisms of the AIM2 inflammasome and IFI16 receptor.
Jin T, Perry A, Jiang J, Smith P, Curry JA, Unterholzner L, Jiang Z, Horvath G, Rathinam VA, Johnstone RW, Hornung V, Latz E, Bowie AG, Fitzgerald KA, Xiao TS
Immunity 36 561-71 (2012)
PMID: 22483801

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116835 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Gamma-interferon-inducible protein 16 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 204 amino acids
Theoretical weight: 22.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16666 (Residues: 571-766; Coverage: 25%)
Gene names: IFI16, IFNGIP1
Sequence domains: HIN-200/IF120x domain
Structure domains: Nucleic acid-binding proteins
DNA (5'-D(*GP*CP*CP*AP*TP*CP*AP*AP*AP*GP*AP*GP*AP*GP*AP*G)-3') Chain: K
Molecule details ›
Chain: K
Length: 16 nucleotides
Theoretical weight: 4.97 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*TP*CP*TP*CP*TP*CP*TP*TP*TP*GP*AP*TP*GP*GP*CP*C)-3') Chain: L
Molecule details ›
Chain: L
Length: 16 nucleotides
Theoretical weight: 4.83 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand FMT 5 x FMT
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: R3
Unit cell:
a: 126.841Å b: 126.841Å c: 163.868Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.176 0.216
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

175 review citations

Activation and regulation of the inflammasomes.
Latz et al. (2013)
174 more

11 mentions without citation

The emerging role of nuclear viral DNA sensors.
Diner et al. (2015)
10 more