› 3rqs

X-ray diffraction
2Å resolution

Crystal Structure of human L-3- Hydroxyacyl-CoA dehydrogenase (EC1.1.1.35) from mitochondria at the resolution 2.0 A, Northeast Structural Genomics Consortium Target HR487, Mitochondrial Protein Partnership

Released:
DOI: 10.2210/pdb3rqs/pdb
PDB entry 3rqs coloured by chain, front view.
PDB entry 3rqs coloured by chain, side view.
PDB entry 3rqs coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kuzin A, Su M, Seetharaman J, Patel P, Xiao R, Ciccosanti C, Shastry R, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG), Mitochondrial Protein Partnership (MPP)

Function and Biology Details

Reaction catalysed: 
(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 36.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16836 (Residues: 1-314; Coverage: 100%)
Gene names: HAD, HAD1, HADH, HADHSC, SCHAD
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 7 x GOL
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P212121
Unit cell:
a: 49.691Å b: 85.761Å c: 165.614Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.204
Expression system: Escherichia coli

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