› 3rsn

X-ray diffraction
2.1Å resolution

Crystal Structure of the N-terminal region of Human Ash2L

Released:
DOI: 10.2210/pdb3rsn/pdb
PDB entry 3rsn coloured by chain, front view.
PDB entry 3rsn coloured by chain, side view.
PDB entry 3rsn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the N-terminal region of human Ash2L shows a winged-helix motif involved in DNA binding.
Chen Y, Wan B, Wang KC, Cao F, Yang Y, Protacio A, Dou Y, Chang HY, Lei M
EMBO Rep 12 797-803 (2011)
PMID: 21660059

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Set1/Ash2 histone methyltransferase complex subunit ASH2 Chain: A
Molecule details ›
Chain: A
Length: 177 amino acids
Theoretical weight: 20.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UBL3 (Residues: 96-271; Coverage: 28%)
Gene names: ASH2L, ASH2L1
Sequence domains: Set1/Ash2 histone methyltransferase complex subunit ASH2-like, WH
Structure domains: DNA primase DNAg catalytic core, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P3121
Unit cell:
a: 49.984Å b: 49.984Å c: 165.519Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.213 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Hijacked in cancer: the KMT2 (MLL) family of methyltransferases.
Rao et al. (2015)
7 more

4 mentions without citation

Crystal structure of the N-terminal region of human Ash2L shows a winged-helix motif involved in DNA binding.
Chen et al. (2011)
3 more