› 3rw6

X-ray diffraction
2.3Å resolution

Structure of nuclear RNA export factor TAP bound to CTE RNA

Released:
DOI: 10.2210/pdb3rw6/pdb
PDB entry 3rw6 coloured by chain, front view.
PDB entry 3rw6 coloured by chain, side view.
PDB entry 3rw6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-function studies of nucleocytoplasmic transport of retroviral genomic RNA by mRNA export factor TAP.
Teplova M, Wohlbold L, Khin NW, Izaurralde E, Patel DJ
Nat Struct Mol Biol 18 990-8 (2011)
PMID: 21822283

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193681 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nuclear RNA export factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 30.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UBU9 (Residues: 96-362; Coverage: 43%)
Gene names: NXF1, TAP
Sequence domains: Tap, RNA-binding
Structure domains:
constitutive transport element(CTE)of Mason-Pfizer monkey virus RNA Chains: F, H
Molecule details ›
Chains: F, H
Length: 62 nucleotides
Theoretical weight: 20.28 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
2 modified residues:
Ligand GTP 2 x GTP
Ligand CCC 2 x CCC

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 55.412Å b: 117.048Å c: 84.773Å
α: 90° β: 106.4° γ: 90°
R-values:
R R work R free
0.206 0.203 0.256
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

The role of TREX in gene expression and disease.
Heath et al. (2016)
5 more

15 mentions without citation

The Nuclear Pore Complex as a Flexible and Dynamic Gate.
Knockenhauer et al. (2016)
14 more