› 3rw9

X-ray diffraction
2Å resolution

Crystal Structure of human Spermidine Synthase in Complex with decarboxylated S-adenosylhomocysteine

Released:
DOI: 10.2210/pdb3rw9/pdb
PDB entry 3rw9 coloured by chain, front view.
PDB entry 3rw9 coloured by chain, side view.
PDB entry 3rw9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Binding and inhibition of human spermidine synthase by decarboxylated S-adenosylhomocysteine.
Sečkutė J, McCloskey DE, Thomas HJ, Secrist JA, Pegg AE, Ealick SE
Protein Sci 20 1836-44 (2011)
PMID: 21898642

Function and Biology Details

Reaction catalysed: 
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148670 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spermidine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 304 amino acids
Theoretical weight: 34.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19623 (Residues: 1-302; Coverage: 100%)
Gene names: SPS1, SRM, SRML1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand DSH 2 x DSH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 58.177Å b: 60.816Å c: 87.021Å
α: 90° β: 108.05° γ: 90°
R-values:
R R work R free
0.213 0.211 0.259
Expression system: Escherichia coli

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Citations

1 review citation

Polyamine-trypanothione pathway: an update.
Ilari et al. (2017)
6 other articles

1 mention without citation

Binding and inhibition of human spermidine synthase by decarboxylated S-adenosylhomocysteine.
Sečkutė et al. (2011)