› 3rwr

X-ray diffraction
3.94Å resolution

Crystal structure of the human XRCC4-XLF complex

Released:
DOI: 10.2210/pdb3rwr/pdb
PDB entry 3rwr coloured by chain, front view.
PDB entry 3rwr coloured by chain, side view.
PDB entry 3rwr coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Andres SN, Junop MS

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171684 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA repair protein XRCC4 Chains: A, B, F, G, J, K, N, P, R, U, V, Y
Molecule details ›
Chains: A, B, F, G, J, K, N, P, R, U, V, Y
Length: 163 amino acids
Theoretical weight: 18.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13426 (Residues: 1-157; Coverage: 47%)
Gene name: XRCC4
Sequence domains: DNA double-strand break repair and V(D)J recombination protein XRCC4
Structure domains:
Non-homologous end-joining factor 1 Chains: D, E, H, I, L, M, O, Q, S, T, W, X
Molecule details ›
Chains: D, E, H, I, L, M, O, Q, S, T, W, X
Length: 230 amino acids
Theoretical weight: 26.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H9Q4 (Residues: 1-224; Coverage: 75%)
Gene names: NHEJ1, XLF
Sequence domains: XLF-Cernunnos, XRcc4-like factor, NHEJ component
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TBR 8 x TBR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 745.379Å b: 149.585Å c: 80.475Å
α: 90° β: 94.72° γ: 90°
R-values:
R R work R free
0.274 0.271 0.326
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

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