› 3rze

X-ray diffraction
3.1Å resolution

Structure of the human histamine H1 receptor in complex with doxepin

Released:
DOI: 10.2210/pdb3rze/pdb
PDB entry 3rze coloured by chain, front view.
PDB entry 3rze coloured by chain, side view.
PDB entry 3rze coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the human histamine H1 receptor complex with doxepin.
Shimamura T, Shiroishi M, Weyand S, Tsujimoto H, Winter G, Katritch V, Abagyan R, Cherezov V, Liu W, Han GW, Kobayashi T, Stevens RC, Iwata S
Nature 475 65-70 (2011)
PMID: 21697825

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132979 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Histamine H1 receptor Chain: A
Molecule details ›
Chain: A
Length: 452 amino acids
Theoretical weight: 52.38 KDa
Source organisms: Expression system: Komagataella pastoris
UniProt:
  • Canonical: P35367 (Residues: 20-221, 377-377, 405-487; Coverage: 59%)
  • Canonical: P00720 (Residues: 2-136, 138-161; Coverage: 97%)
Gene names: E, HRH1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand 5EH 1 x 5EH
Ligand D7V 1 x D7V
Ligand PO4 3 x PO4
Ligand OLC 1 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: I422
Unit cell:
a: 88.144Å b: 88.144Å c: 331.654Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.249
Expression system: Komagataella pastoris

Quick links

Citations

109 review citations

Molecular signatures of G-protein-coupled receptors.
Venkatakrishnan et al. (2013)
108 more

172 mentions without citation

Diversity and modularity of G protein-coupled receptor structures.
Katritch et al. (2012)
171 more