› 3rzx

X-ray diffraction
2.61Å resolution

Mouse importin alpha-Ku70 NLS peptide complex

Released:
DOI: 10.2210/pdb3rzx/pdb
PDB entry 3rzx coloured by chain, front view.
PDB entry 3rzx coloured by chain, side view.
PDB entry 3rzx coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of importin-α-mediated nuclear transport for Ku70 and Ku80.
Takeda AA, de Barros AC, Chang CW, Kobe B, Fontes MR
J Mol Biol 412 226-34 (2011)
PMID: 21806995

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146402 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 510 amino acids
Theoretical weight: 55.33 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 70-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
X-ray repair cross-complementing protein 6 Chain: B
Molecule details ›
Chain: B
Length: 22 amino acids
Theoretical weight: 2.47 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P12956 (Residues: 537-558; Coverage: 4%)
Gene names: G22P1, XRCC6

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 78.518Å b: 89.996Å c: 100.142Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.223
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Structural Biology and Regulation of Protein Import into the Nucleus.
Christie et al. (2016)
4 more

6 mentions without citation

The C-terminal 18 Amino Acid Region of Dengue Virus NS5 Regulates its Subcellular Localization and Contains a Conserved Arginine Residue Essential for Infectious Virus Production.
Tay et al. (2016)
5 more