› 3s24

X-ray diffraction
3.01Å resolution

Crystal structure of human mRNA guanylyltransferase

Released:
DOI: 10.2210/pdb3s24/pdb
PDB entry 3s24 coloured by chain, front view.
PDB entry 3s24 coloured by chain, side view.
PDB entry 3s24 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the guanylyltransferase domain of human mRNA capping enzyme.
Chu C, Das K, Tyminski JR, Bauman JD, Guan R, Qiu W, Montelione GT, Arnold E, Shatkin AJ
Proc Natl Acad Sci U S A 108 10104-8 (2011)
PMID: 21636784

Function and Biology Details

Reactions catalysed: 
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
A 5'-triphospho-[mRNA] + H(2)O = a 5'-diphospho-[mRNA] + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130222 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mRNA-capping enzyme Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 347 amino acids
Theoretical weight: 39.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60942 (Residues: 229-567; Coverage: 57%)
Gene names: CAP1A, RNGTT
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 92.121Å b: 104.658Å c: 149.571Å
α: 90° β: 94.95° γ: 90°
R-values:
R R work R free
0.26 0.258 0.296
Expression system: Escherichia coli

Quick links

Citations

11 review citations

mRNA capping: biological functions and applications.
Ramanathan et al. (2016)
10 more

5 mentions without citation

X-ray structure and activities of an essential Mononegavirales L-protein domain.
Paesen et al. (2015)
4 more