› 3s8e

X-ray diffraction
2.88Å resolution

Phosphorylation regulates assembly of the caspase-6 substrate-binding groove

Released:
DOI: 10.2210/pdb3s8e/pdb
PDB entry 3s8e coloured by chain, front view.
PDB entry 3s8e coloured by chain, side view.
PDB entry 3s8e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Phosphorylation regulates assembly of the caspase-6 substrate-binding groove.
Velázquez-Delgado EM, Hardy JA
Structure 20 742-51 (2012)
PMID: 22483120

Function and Biology Details

Reaction catalysed: 
Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-|-
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157270 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Caspase-6 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 277 amino acids
Theoretical weight: 31.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55212 (Residues: 24-293; Coverage: 92%)
Gene names: CASP6, MCH2
Sequence domains: Caspase domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 81.671Å b: 163.662Å c: 89.015Å
α: 90° β: 94.17° γ: 90°
R-values:
R R work R free
0.218 0.216 0.256
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Post-translational Modification of Caspases: The Other Side of Apoptosis Regulation.
Zamaraev et al. (2017)
6 more

2 mentions without citation

Phosphorylation regulates assembly of the caspase-6 substrate-binding groove.
Velázquez-Delgado et al. (2012)
1 more