› 3s9k

X-ray diffraction
2.35Å resolution

Crystal structure of the Itk SH2 domain.

Released:
DOI: 10.2210/pdb3s9k/pdb
PDB entry 3s9k coloured by chain, front view.
PDB entry 3s9k coloured by chain, side view.
PDB entry 3s9k coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of the interleukin-2 tyrosine kinase Src homology 2 domain; comparison between X-ray and NMR-derived structures.
Joseph RE, Ginder ND, Hoy JA, Nix JC, Fulton DB, Honzatko RB, Andreotti AH
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 145-53 (2012)
PMID: 22297986

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-169911 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase ITK/TSK Chain: A
Molecule details ›
Chain: A
Length: 118 amino acids
Theoretical weight: 13.54 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03526 (Residues: 236-344; Coverage: 17%)
Gene names: Emt, Itk, Tlk, Tsk
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

1 bound ligand:
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: I222
Unit cell:
a: 53.64Å b: 57.36Å c: 83.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.233 0.283
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Reining in BTK: Interdomain Interactions and Their Importance in the Regulatory Control of BTK.
Kueffer et al. (2021)
2 other articles

2 mentions without citation

Structural insights into the intertwined dimer of fyn SH2.
Huculeci et al. (2015)
1 more