3sc0

X-ray diffraction
1.95Å resolution

Crystal Structure of MMACHC (1-238), a human B12 processing enzyme, complexed with MethylCobalamin

Released:
Source organism: Homo sapiens

Function and Biology Details

Reactions catalysed:
2 cob(II)alamin-[cyanocobalamin reductase] + 2 hydrogen cyanide + NADP(+) = 2 cyanocob(III)alamin + 2 [cyanocobalamin reductase] + NADPH
An alkylcobalamin + [alkylcobalamin reductase] + glutathione = cob(I)alamin-[alkylcobalamin reductase] + an S-alkylglutathione
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
PDBe Complex ID:
PDB-CPX-195250 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyanocobalamin reductase / alkylcobalamin dealkylase Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 27.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y4U1 (Residues: 1-238; Coverage: 84%)
Gene name: MMACHC
Sequence domains: Methylmalonic aciduria and homocystinuria type C family

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P6222
Unit cell:
a: 105.955Å b: 105.955Å c: 84.253Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.186 0.244
Expression system: Escherichia coli