› 3sfv

X-ray diffraction
1.73Å resolution

Crystal structure of the GDP-bound Rab1a S25N mutant in complex with the coiled-coil domain of LidA from Legionella pneumophila

Released:
DOI: 10.2210/pdb3sfv/pdb
PDB entry 3sfv coloured by chain, front view.
PDB entry 3sfv coloured by chain, side view.
PDB entry 3sfv coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into a unique Legionella pneumophila effector LidA recognizing both GDP and GTP bound Rab1 in their active state.
Cheng W, Yin K, Lu D, Li B, Zhu D, Chen Y, Zhang H, Xu S, Chai J, Gu L
PLoS Pathog 8 e1002528 (2012)
PMID: 22416225

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158706 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related protein Rab-1A Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 20.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62820 (Residues: 1-176; Coverage: 86%)
Gene names: RAB1, RAB1A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
LidA protein, substrate of the Dot/Icm system Chain: B
Molecule details ›
Chain: B
Length: 344 amino acids
Theoretical weight: 40.2 KDa
Source organism: Legionella pneumophila str. Corby
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 154.314Å b: 54.673Å c: 61.699Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.228
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Formation of a pathogen vacuole according to Legionella pneumophila: how to kill one bird with many stones.
Finsel et al. (2015)
10 more

2 mentions without citation

Structural insights into a unique Legionella pneumophila effector LidA recognizing both GDP and GTP bound Rab1 in their active state.
Cheng et al. (2012)
1 more