› 3sg5

X-ray diffraction
1.9Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144018 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1; Green fluorescent protein; Myosin light chain kinase, smooth muscle, deglutamylated form Chain: A
Molecule details ›
Chain: A
Length: 448 amino acids
Theoretical weight: 50.53 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P11799 (Residues: 1731-1749, 1752-1763; Coverage: 2%)
  • Canonical: P42212 (Residues: 2-144, 191-192; Coverage: 61%)
  • Canonical: P0DP29 (Residues: 3-42, 45-149; Coverage: 97%)
Gene names: CaMI, Calm, Calm1, Cam, Cam1, GFP, Mylk
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand SO4 4 x SO4
Ligand GOL 1 x GOL
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 128.567Å b: 46.005Å c: 67.365Å
α: 90° β: 100.19° γ: 90°
R-values:
R R work R free
0.182 0.18 0.226
Expression system: Escherichia coli

Quick links

Citations

150 review citations

Genetically encoded indicators of neuronal activity.
Lin et al. (2016)
149 more

1 mention without citation

Optimization of a GCaMP calcium indicator for neural activity imaging.
Akerboom et al. (2012)