› 3sg7

X-ray diffraction
1.9Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144017 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1; Green fluorescent protein; Myosin light chain kinase, smooth muscle, deglutamylated form Chain: A
Molecule details ›
Chain: A
Length: 448 amino acids
Theoretical weight: 50.58 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P11799 (Residues: 1731-1749, 1752-1763; Coverage: 2%)
  • Canonical: P42212 (Residues: 2-144, 151-170, 191-192; Coverage: 69%)
  • Canonical: P0DP29 (Residues: 23-42, 45-149; Coverage: 84%)
Gene names: CaMI, Calm, Calm1, Cam, Cam1, GFP, Mylk
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 57.449Å b: 61.531Å c: 123.968Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.23
Expression system: Escherichia coli

Quick links

Citations

150 review citations

Genetically encoded indicators of neuronal activity.
Lin et al. (2016)
149 more

3 mentions without citation

Optimization of a GCaMP calcium indicator for neural activity imaging.
Akerboom et al. (2012)
2 more