› 3sob

X-ray diffraction
1.9Å resolution

The structure of the first YWTD beta propeller domain of LRP6 in complex with a FAB

Released:
DOI: 10.2210/pdb3sob/pdb
PDB entry 3sob coloured by chain, front view.
PDB entry 3sob coloured by chain, side view.
PDB entry 3sob coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Wnt antagonists bind through a short peptide to the first β-propeller domain of LRP5/6.
Bourhis E, Wang W, Tam C, Hwang J, Zhang Y, Spittler D, Huang OW, Gong Y, Estevez A, Zilberleyb I, Rouge L, Chiu C, Wu Y, Costa M, Hannoush RN, Franke Y, Cochran AG
Structure 19 1433-42 (2011)
PMID: 21944579

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208149 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
antibody light chain Chain: L
Molecule details ›
Chain: L
Length: 237 amino acids
Theoretical weight: 25.82 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Structure domains: Immunoglobulins
Low-density lipoprotein receptor-related protein 6 Chain: B
Molecule details ›
Chain: B
Length: 316 amino acids
Theoretical weight: 35.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75581 (Residues: 20-335; Coverage: 20%)
Gene name: LRP6
Sequence domains:
Structure domains:
antibody heavy chain Chain: H
Molecule details ›
Chain: H
Length: 256 amino acids
Theoretical weight: 27.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 167.037Å b: 83.363Å c: 62.844Å
α: 90° β: 111.8° γ: 90°
R-values:
R R work R free
0.176 0.174 0.212
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

31 review citations

WNT signaling in bone homeostasis and disease: from human mutations to treatments.
Baron et al. (2013)
30 more

9 mentions without citation

Bapineuzumab captures the N-terminus of the Alzheimer's disease amyloid-beta peptide in a helical conformation.
Miles et al. (2013)
8 more