› 3spa

X-ray diffraction
2.5Å resolution

Crystal Structure of Human Mitochondrial RNA Polymerase

Released:
DOI: 10.2210/pdb3spa/pdb
PDB entry 3spa coloured by chain, front view.
PDB entry 3spa coloured by chain, side view.
PDB entry 3spa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human mitochondrial RNA polymerase.
Ringel R, Sologub M, Morozov YI, Litonin D, Cramer P, Temiakov D
Nature 478 269-73 (2011)
PMID: 21947009

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126266 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA-directed RNA polymerase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 1134 amino acids
Theoretical weight: 128.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00411 (Residues: 105-1230; Coverage: 92%)
Gene name: POLRMT
Sequence domains:
Nonamer peptide Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 784 Da
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand CL 5 x CL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: I41
Unit cell:
a: 211.258Å b: 211.258Å c: 60.461Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.231
Expression system: Escherichia coli

Quick links

Citations

32 review citations

Pentatricopeptide repeat proteins in plants.
Barkan et al. (2014)
31 more

20 mentions without citation

Replicating animal mitochondrial DNA.
McKinney et al. (2013)
19 more