› 3sqe

X-ray diffraction
1.9Å resolution

Crystal structure of prethrombin-2 mutant S195A in the alternative form

Released:
DOI: 10.2210/pdb3sqe/pdb
PDB entry 3sqe coloured by chain, front view.
PDB entry 3sqe coloured by chain, side view.
PDB entry 3sqe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of prethrombin-2 reveal alternative conformations under identical solution conditions and the mechanism of zymogen activation.
Pozzi N, Chen Z, Zapata F, Pelc LA, Barranco-Medina S, Di Cera E
Biochemistry 50 10195-202 (2011)
PMID: 22049947

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thrombin heavy chain Chain: E
Molecule details ›
Chain: E
Length: 290 amino acids
Theoretical weight: 33.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00734 (Residues: 333-622; Coverage: 49%)
Gene name: F2
Sequence domains:
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 44.378Å b: 58.055Å c: 52.88Å
α: 90° β: 98.24° γ: 90°
R-values:
R R work R free
0.173 0.171 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Mechanisms of ligand binding.
Di Cera E. (2020)
1 more

4 mentions without citation

Crystal structures of prethrombin-2 reveal alternative conformations under identical solution conditions and the mechanism of zymogen activation.
Pozzi et al. (2011)
3 more