› 3sqp

X-ray diffraction
2.21Å resolution

Structure of human glutathione reductase complexed with pyocyanin, an agent with antimalarial activity

Released:
DOI: 10.2210/pdb3sqp/pdb
PDB entry 3sqp coloured by chain, front view.
PDB entry 3sqp coloured by chain, side view.
PDB entry 3sqp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The bacterial redox signaller pyocyanin as an antiplasmodial agent: comparisons with its thioanalog methylene blue.
Kasozi DM, Gromer S, Adler H, Zocher K, Rahlfs S, Wittlin S, Fritz-Wolf K, Schirmer RH, Becker K
Redox Rep 16 154-65 (2011)
PMID: 21888766

Function and Biology Details

Reaction catalysed: 
2 glutathione + NADP(+) = glutathione disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione reductase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 478 amino acids
Theoretical weight: 51.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00390 (Residues: 45-522; Coverage: 92%)
Gene names: GLUR, GRD1, GSR
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
3 bound ligands:
Ligand SO4 4 x SO4
Ligand 3J8 2 x 3J8
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 62.08Å b: 67.37Å c: 78.86Å
α: 68.98° β: 66.81° γ: 62.54°
R-values:
R R work R free
0.146 0.144 0.182
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The structural biology of phenazine biosynthesis.
Blankenfeldt et al. (2014)
3 more

4 mentions without citation

Thioredoxin reductase and its inhibitors.
Saccoccia et al. (2014)
3 more