› 3srh

X-ray diffraction
2.6Å resolution

Human M2 pyruvate kinase

Released:
DOI: 10.2210/pdb3srh/pdb
PDB entry 3srh coloured by chain, front view.
PDB entry 3srh coloured by chain, side view.
PDB entry 3srh coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Morgan HP, O'Reilly F, Palmer R, McNae IW, Nowicki MW, Wear MA, Fothergill-Gilmore LA, Walkinshaw MD

Function and Biology Details

Reactions catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146985 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase PKM Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 551 amino acids
Theoretical weight: 60.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14618 (Residues: 1-531; Coverage: 100%)
Gene names: OIP3, PK2, PK3, PKM, PKM2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 81.2Å b: 154.31Å c: 98.59Å
α: 90° β: 105.59° γ: 90°
R-values:
R R work R free
0.219 0.217 0.27
Expression system: Escherichia coli BL21(DE3)

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