› 3sy2

X-ray diffraction
3.27Å resolution

Crystal structure of the Salmonella E3 ubiquitin ligase SopA in complex with the human E2 UbcH7

Released:
DOI: 10.2210/pdb3sy2/pdb
PDB entry 3sy2 coloured by chain, front view.
PDB entry 3sy2 coloured by chain, side view.
PDB entry 3sy2 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structures of two bacterial HECT-like E3 ligases in complex with a human E2 reveal atomic details of pathogen-host interactions.
Lin DY, Diao J, Chen J
Proc Natl Acad Sci U S A 109 1925-30 (2012)
PMID: 22308380

Function and Biology Details

Reactions catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159273 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase SopA Chains: A, B
Molecule details ›
Chains: A, B
Length: 621 amino acids
Theoretical weight: 69.24 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZNR3 (Residues: 165-782; Coverage: 79%)
Gene names: STM2066, sopA
Sequence domains:
Structure domains:
Ubiquitin-conjugating enzyme E2 L3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 156 amino acids
Theoretical weight: 18.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P68036 (Residues: 1-154; Coverage: 100%)
Gene names: UBCE7, UBCH7, UBE2L3
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 74.12Å b: 118.363Å c: 241.671Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.211 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Ubiquitin Ligases: Structure, Function, and Regulation.
Zheng et al. (2017)
18 more

2 mentions without citation

Crystal structures of two bacterial HECT-like E3 ligases in complex with a human E2 reveal atomic details of pathogen-host interactions.
Lin et al. (2012)
1 more