› 3t1n

X-ray diffraction
2.6Å resolution

Structure of human MICROCEPHALIN (MCPH1) TANDEM BRCT domains in complex with a CDC27 phosphopeptide

Released:
DOI: 10.2210/pdb3t1n/pdb
PDB entry 3t1n coloured by chain, front view.
PDB entry 3t1n coloured by chain, side view.
PDB entry 3t1n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for the association of microcephalin (MCPH1) protein with the cell division cycle protein 27 (Cdc27) subunit of the anaphase-promoting complex.
Singh N, Wiltshire TD, Thompson JR, Mer G, Couch FJ
J Biol Chem 287 2854-62 (2012)
PMID: 22139841

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151684 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Microcephalin Chains: A, B
Molecule details ›
Chains: A, B
Length: 199 amino acids
Theoretical weight: 21.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8NEM0 (Residues: 640-835; Coverage: 24%)
Gene name: MCPH1
Structure domains: BRCT domain
Cell division cycle protein 27 homolog Chains: C, D
Molecule details ›
Chains: C, D
Length: 4 amino acids
Theoretical weight: 576 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P30260 (Residues: 821-824; Coverage: 1%)
Gene names: ANAPC3, CDC27, D0S1430E, D17S978E

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P1
Unit cell:
a: 38.201Å b: 46.506Å c: 55.322Å
α: 86.06° β: 70.69° γ: 89.67°
R-values:
R R work R free
0.232 0.231 0.262
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

MCPH1: a window into brain development and evolution.
Pulvers et al. (2015)
7 more

2 mentions without citation

Molecular basis for the association of microcephalin (MCPH1) protein with the cell division cycle protein 27 (Cdc27) subunit of the anaphase-promoting complex.
Singh et al. (2012)
1 more