› 3t2n

X-ray diffraction
2.55Å resolution

Human hepsin protease in complex with the Fab fragment of an inhibitory antibody

Released:
DOI: 10.2210/pdb3t2n/pdb
PDB entry 3t2n coloured by chain, front view.
PDB entry 3t2n coloured by chain, side view.
PDB entry 3t2n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric antibody inhibition of human hepsin protease.
Koschubs T, Dengl S, Dürr H, Kaluza K, Georges G, Hartl C, Jennewein S, Lanzendörfer M, Auer J, Stern A, Huang KS, Packman K, Gubler U, Kostrewa D, Ries S, Hansen S, Kohnert U, Cramer P, Mundigl O
Biochem J 442 483-94 (2012)
PMID: 22132769

Function and Biology Details

Reaction catalysed: 
Cleavage after basic amino-acid residues, with Arg strongly preferred to Lys
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209273 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Serine protease hepsin catalytic chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 372 amino acids
Theoretical weight: 40.49 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P05981 (Residues: 46-417; Coverage: 89%)
Gene names: HPN, TMPRSS1
Sequence domains:
Structure domains:
Antibody, Fab fragment, Heavy Chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 225 amino acids
Theoretical weight: 23.97 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins
Antibody, Fab fragment, Light Chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 215 amino acids
Theoretical weight: 22.88 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 62.98Å b: 66.58Å c: 108.33Å
α: 88.71° β: 94.3° γ: 104.53°
R-values:
R R work R free
0.244 0.242 0.275
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

5 review citations

The role of type II transmembrane serine protease-mediated signaling in cancer.
Tanabe et al. (2017)
4 more

5 mentions without citation

Computers and viral diseases. Preliminary bioinformatics studies on the design of a synthetic vaccine and a preventative peptidomimetic antagonist against the SARS-CoV-2 (2019-nCoV, COVID-19) coronavirus.
Robson B. (2020)
4 more