› 3t3s

X-ray diffraction
3Å resolution

Human Cytochrome P450 2A13 in complex with Pilocarpine

Released:
DOI: 10.2210/pdb3t3s/pdb
PDB entry 3t3s coloured by chain, front view.
PDB entry 3t3s coloured by chain, side view.
PDB entry 3t3s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural comparison of cytochromes P450 2A6, 2A13, and 2E1 with pilocarpine.
DeVore NM, Meneely KM, Bart AG, Stephens ES, Battaile KP, Scott EE
FEBS J 279 1621-31 (2012)
PMID: 22051186

Function and Biology Details

Reaction catalysed: 
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 2A13 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 476 amino acids
Theoretical weight: 54.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16696 (Residues: 31-494; Coverage: 94%)
Gene name: CYP2A13
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 8 x HEM
1 bound ligand:
Ligand 9PL 6 x 9PL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P1
Unit cell:
a: 71.501Å b: 119.862Å c: 154.871Å
α: 101.01° β: 101.72° γ: 93.59°
R-values:
R R work R free
0.218 0.213 0.303
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structural diversity of eukaryotic membrane cytochrome p450s.
Johnson et al. (2013)
3 more

18 mentions without citation

The rheology of direct and suspended extrusion bioprinting.
Cooke et al. (2021)
17 more