› 3t4a

X-ray diffraction
3.4Å resolution

Structure of a truncated form of Staphylococcal Complement Inhibitor B bound to human C3c at 3.4 Angstrom resolution

Released:
DOI: 10.2210/pdb3t4a/pdb
PDB entry 3t4a coloured by chain, front view.
PDB entry 3t4a coloured by chain, side view.
PDB entry 3t4a coloured by chain, top view.
Source organisms:
Primary publication:
Diversity in the C3b [corrected] contact residues and tertiary structures of the staphylococcal complement inhibitor (SCIN) protein family.
Garcia BL, Summers BJ, Lin Z, Ramyar KX, Ricklin D, Kamath DV, Fu ZQ, Lambris JD, Geisbrecht BV
J Biol Chem 287 628-640 (2012)
PMID: 22086928

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102008 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Complement C3 beta chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 645 amino acids
Theoretical weight: 71.39 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01024 (Residues: 23-667; Coverage: 39%)
Gene names: C3, CPAMD1
Sequence domains:
Structure domains:
Complement C3c alpha' chain fragment 1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 206 amino acids
Theoretical weight: 23.62 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01024 (Residues: 749-954; Coverage: 13%)
Gene names: C3, CPAMD1
Sequence domains:
Structure domains:
Complement C3c alpha' chain fragment 2 Chains: C, F
Molecule details ›
Chains: C, F
Length: 343 amino acids
Theoretical weight: 39.54 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01024 (Residues: 1321-1663; Coverage: 21%)
Gene names: C3, CPAMD1
Sequence domains:
Structure domains:
Staphylococcal complement inhibitor Chains: G, H
Molecule details ›
Chains: G, H
Length: 73 amino acids
Theoretical weight: 8.21 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3JZ23 (Residues: 49-116; Coverage: 72%)
Gene name: SAV1159
Sequence domains: Staphylococcal complement inhibitor SCIN
Structure domains: Substrate Binding Domain Of Dnak; Chain:A; Domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 103.304Å b: 165.436Å c: 203.125Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.229 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Novel Evasion Mechanisms of the Classical Complement Pathway.
Garcia et al. (2016)
1 more

5 mentions without citation

Complement component C3: A structural perspective and potential therapeutic implications.
Geisbrecht et al. (2022)
4 more