› 3t4n

X-ray diffraction
2.3Å resolution

Structure of the regulatory fragment of Saccharomyces cerevisiae AMPK in complex with ADP

Released:
DOI: 10.2210/pdb3t4n/pdb
PDB entry 3t4n coloured by chain, front view.
PDB entry 3t4n coloured by chain, side view.
PDB entry 3t4n coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
ADP regulates SNF1, the Saccharomyces cerevisiae homolog of AMP-activated protein kinase.
Mayer FV, Heath R, Underwood E, Sanders MJ, Carmena D, McCartney RR, Leiper FC, Xiao B, Jing C, Walker PA, Haire LF, Ogrodowicz R, Martin SR, Schmidt MC, Gamblin SJ, Carling D
Cell Metab 14 707-14 (2011)
PMID: 22019086

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139224 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Carbon catabolite-derepressing protein kinase Chain: A
Molecule details ›
Chain: A
Length: 179 amino acids
Theoretical weight: 20.45 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P06782 (Residues: 457-633; Coverage: 28%)
Gene names: CAT1, CCR1, D8035.20, GLC2, PAS14, SNF1, YDR477W
Sequence domains: Adenylate sensor of SNF1-like protein kinase
Structure domains: Kinase associated domain 1, KA1
SNF1 protein kinase subunit beta-2 Chain: B
Molecule details ›
Chain: B
Length: 113 amino acids
Theoretical weight: 13.04 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P34164 (Residues: 303-415; Coverage: 27%)
Gene names: G1155, SIP2, SPM2, YGL208W
Sequence domains: 5'-AMP-activated protein kinase beta subunit, interaction domain
Structure domains: N-terminal domain of TfIIb
5'-AMP-activated protein kinase subunit gamma Chain: C
Molecule details ›
Chain: C
Length: 323 amino acids
Theoretical weight: 36.51 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P12904 (Residues: 2-322; Coverage: 100%)
Gene names: CAT3, SCI1, SNF4, YGL115W
Sequence domains: CBS domain
Structure domains: CBS-domain

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2221
Unit cell:
a: 88.599Å b: 251.214Å c: 79.589Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.268
Expression system: Escherichia coli

Quick links

Citations

29 review citations

AMPK: a nutrient and energy sensor that maintains energy homeostasis.
Hardie et al. (2012)
28 more

1 mention without citation

N-terminal domain of nuclear IL-1α shows structural similarity to the C-terminal domain of Snf1 and binds to the HAT/core module of the SAGA complex.
Zamostna et al. (2012)