› 3t7k

X-ray diffraction
2.03Å resolution

Complex structure of Rtt107p and phosphorylated histone H2A

Released:
DOI: 10.2210/pdb3t7k/pdb
PDB entry 3t7k coloured by chain, front view.
PDB entry 3t7k coloured by chain, side view.
PDB entry 3t7k coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure of C-terminal tandem BRCT repeats of Rtt107 protein reveals critical role in interaction with phosphorylated histone H2A during DNA damage repair.
Li X, Liu K, Li F, Wang J, Huang H, Wu J, Shi Y
J Biol Chem 287 9137-46 (2012)
PMID: 22262834

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138262 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Regulator of Ty1 transposition protein 107 Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 29.39 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38850 (Residues: 820-1070; Coverage: 24%)
Gene names: ESC4, RTT107, YHR154W
Sequence domains:
Structure domains: BRCT domain
Histone H2A.1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 8 amino acids
Theoretical weight: 928 Da
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P04911 (Residues: 125-132; Coverage: 6%)
Gene names: H2A1, HTA1, SPT11, YD9934.10, YDR225W

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 34.517Å b: 59.255Å c: 85.89Å
α: 78.36° β: 89.68° γ: 73.17°
R-values:
R R work R free
0.22 0.218 0.258
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

Perceiving the epigenetic landscape through histone readers.
Musselman et al. (2012)
10 more

1 mention without citation

Structure of C-terminal tandem BRCT repeats of Rtt107 protein reveals critical role in interaction with phosphorylated histone H2A during DNA damage repair.
Li et al. (2012)