Function and Biology Details
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Proliferating cell nuclear antigen, PCNA
- Ubiquitin domain
- Ubiquitin-like domain
- Proliferating cell nuclear antigen, PCNA, conserved site
- DNA clamp superfamily
- Proliferating cell nuclear antigen, PCNA, C-terminal
- Proliferating cell nuclear antigen, PCNA, N-terminal
- Ubiquitin conserved site
1 more domain
Structure analysis Details
Assembly composition:
hetero pentamer (preferred)
Assembly name:
PCNA homotrimer and Ubiquitin (preferred)
PDBe Complex ID:
PDB-CPX-146048 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferating cell nuclear antigen
Molecule details ›
Chains: A, B, C
Length: 261 amino acids
Theoretical weight: 28.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli DH5[alpha]
UniProt:
Sequence domains:
Length: 261 amino acids
Theoretical weight: 28.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli DH5[alpha]
UniProt:
- Canonical:
P12004 (Residues: 1-261; Coverage: 100%)
Sequence domains:
- Proliferating cell nuclear antigen, N-terminal domain
- Proliferating cell nuclear antigen, C-terminal domain
Ubiquitin
Molecule details ›
Chains: D, E
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: P62979 (Residues: 1-76; Coverage: 49%)
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 19-ID
Spacegroup:
P43212
Expression systems:
- Escherichia coli DH5[alpha]
- Escherichia coli
