› 3tdk

X-ray diffraction
2.8Å resolution

Crystal Structure of Human UDP-Glucose Dehydrogenase

Released:
DOI: 10.2210/pdb3tdk/pdb
PDB entry 3tdk coloured by chain, front view.
PDB entry 3tdk coloured by chain, side view.
PDB entry 3tdk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of cooperativity in human UDP-glucose dehydrogenase.
Rajakannan V, Lee HS, Chong SH, Ryu HB, Bae JY, Whang EY, Huh JW, Cho SW, Kang LW, Choe H, Robinson RC
PLoS One 6 e25226 (2011)
PMID: 21984906

Function and Biology Details

Reaction catalysed: 
UDP-glucose + 2 NAD(+) + H(2)O = UDP-glucuronate + 2 NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 6-dehydrogenase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 487 amino acids
Theoretical weight: 54.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60701 (Residues: 1-487; Coverage: 99%)
Gene name: UGDH
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 12 x NAD
1 bound ligand:
Ligand UPG 12 x UPG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P212121
Unit cell:
a: 173.132Å b: 191.177Å c: 225.807Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.23 0.261
Expression system: Escherichia coli

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Citations

2 review citations

Reconstruction of an in vitro niche for the transition from intervertebral disc development to nucleus pulposus regeneration.
Shoukry et al. (2013)
1 more

3 mentions without citation

Structural basis of cooperativity in human UDP-glucose dehydrogenase.
Rajakannan et al. (2011)
2 more