Function and Biology Details
Reaction catalysed:
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-158199 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DCN1-like protein 1
Molecule details ›
Chains: A, B
Length: 200 amino acids
Theoretical weight: 23.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Cullin binding
Structure domains: EF-hand
Length: 200 amino acids
Theoretical weight: 23.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
Q96GG9 (Residues: 62-259; Coverage: 76%)
Sequence domains: Cullin binding
Structure domains: EF-hand
Cullin-1
Molecule details ›
Chains: C, D
Length: 77 amino acids
Theoretical weight: 9.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Cullin protein neddylation domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Length: 77 amino acids
Theoretical weight: 9.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: Q13616 (Residues: 702-776; Coverage: 10%)
Sequence domains: Cullin protein neddylation domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
NEDD8-conjugating enzyme Ubc12
Molecule details ›
Chains: E, F
Length: 13 amino acids
Theoretical weight: 1.55 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
Length: 13 amino acids
Theoretical weight: 1.55 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
- Canonical: P61081 (Residues: 1-12; Coverage: 7%)
