› 3teg

X-ray diffraction
2.2Å resolution

Bacterial and Eukaryotic Phenylalanyl-tRNA Synthetases Catalyze Misaminoacylation of tRNAPhe with 3,4-Dihydroxy-L-Phenylalanine (L-Dopa)

Released:
DOI: 10.2210/pdb3teg/pdb
PDB entry 3teg coloured by chain, front view.
PDB entry 3teg coloured by chain, side view.
PDB entry 3teg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Bacterial and eukaryotic phenylalanyl-tRNA synthetases catalyze misaminoacylation of tRNA(Phe) with 3,4-dihydroxy-L-phenylalanine.
Moor N, Klipcan L, Safro MG
Chem Biol 18 1221-9 (2011)
PMID: 22035791

Function and Biology Details

Reaction catalysed: 
ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131898 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine--tRNA ligase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 48.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95363 (Residues: 38-451; Coverage: 92%)
Gene names: FARS1, FARS2, HSPC320
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand DAH 1 x DAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 55.185Å b: 90.587Å c: 96.076Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.22
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Expanding the repertoire of L-DOPA's actions: A comprehensive review of its functional neurochemistry.
De Deurwaerdère et al. (2017)
2 more

4 mentions without citation

Development and Testing of Force Field Parameters for Phenylalanine and Tyrosine Derivatives.
Wang et al. (2020)
3 more