› 3tf2

X-ray diffraction
2.1Å resolution

Crystal structure of the cap free human translation initiation factor eIF4E

Released:
DOI: 10.2210/pdb3tf2/pdb
PDB entry 3tf2 coloured by chain, front view.
PDB entry 3tf2 coloured by chain, side view.
PDB entry 3tf2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the allosteric effects of 4EBP1 on the eukaryotic translation initiation factor eIF4E.
Siddiqui N, Tempel W, Nedyalkova L, Volpon L, Wernimont AK, Osborne MJ, Park HW, Borden KL
J Mol Biol 415 781-92 (2012)
PMID: 22178476

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139166 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 4E Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 217 amino acids
Theoretical weight: 25.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06730 (Residues: 1-217; Coverage: 100%)
Gene names: EIF4E, EIF4EL1, EIF4F
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e

Ligands and Environments

1 bound ligand:
Ligand UNX 14 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 39.236Å b: 56.739Å c: 100.31Å
α: 103.61° β: 91.36° γ: 110.14°
R-values:
R R work R free
0.217 0.215 0.26
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Eukaryotic initiation factor 4E-binding protein 1 (4E-BP1): a master regulator of mRNA translation involved in tumorigenesis.
Musa et al. (2016)
7 more

3 mentions without citation

Crystal structure of a minimal eIF4E-Cup complex reveals a general mechanism of eIF4E regulation in translational repression.
Kinkelin et al. (2012)
2 more