› 3th5

X-ray diffraction
2.3Å resolution

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158894 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related C3 botulinum toxin substrate 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 204 amino acids
Theoretical weight: 22.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63000 (Residues: 2-177; Coverage: 92%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GNP 2 x GNP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 40.925Å b: 97.851Å c: 51.733Å
α: 90° β: 96.59° γ: 90°
R-values:
R R work R free
0.231 0.229 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

247 review citations

Drugging the undruggable RAS: Mission possible?
Cox et al. (2014)
246 more

25 mentions without citation

Rho GTPases, oxidation, and cell redox control.
Hobbs et al. (2014)
24 more