3thw

X-ray diffraction
3.09Å resolution

Human MutSbeta complexed with an IDL of 4 bases (Loop4) and ADP

Released:
Source organism: Homo sapiens
Primary publication:
Mechanism of mismatch recognition revealed by human MutSβ bound to unpaired DNA loops.
Nat Struct Mol Biol 19 72-8 (2011)
PMID: 22179786

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-116934 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
DNA mismatch repair protein Msh2 Chain: A
Molecule details ›
Chain: A
Length: 934 amino acids
Theoretical weight: 104.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43246 (Residues: 1-934; Coverage: 100%)
Gene name: MSH2
Sequence domains:
Structure domains:
DNA mismatch repair protein Msh3 Chain: B
Molecule details ›
Chain: B
Length: 918 amino acids
Theoretical weight: 104.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P20585 (Residues: 219-1134; Coverage: 81%)
Gene names: DUC1, DUG, MSH3
Sequence domains:
Structure domains:
DNA Loop4 hairpin Chain: D
Molecule details ›
Chain: D
Length: 53 nucleotides
Theoretical weight: 16.33 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 105.84Å b: 116.13Å c: 180.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.226 0.287
Expression system: Spodoptera frugiperda