› 3tix

X-ray diffraction
2.9Å resolution

Crystal structure of the Chp1-Tas3 complex core

Released:
DOI: 10.2210/pdb3tix/pdb
PDB entry 3tix coloured by chain, front view.
PDB entry 3tix coloured by chain, side view.
PDB entry 3tix coloured by chain, top view.
Source organisms:
Primary publication:
The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS.
Schalch T, Job G, Shanker S, Partridge JF, Joshua-Tor L
Nat Struct Mol Biol 18 1351-7 (2011)
PMID: 22081013

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131723 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA-induced transcriptional silencing complex protein tas3; Ubiquitin-like protein SMT3 Chains: A, C
Molecule details ›
Chains: A, C
Length: 207 amino acids
Theoretical weight: 23.33 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q12306 (Residues: 3-98; Coverage: 95%)
  • Canonical: O94687 (Residues: 9-83; Coverage: 14%)
Gene names: D9719.15, SMT3, SPBC83.03c, YDR510W, tas3
Sequence domains: Ubiquitin family
Structure domains:
Chromo domain-containing protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 458 amino acids
Theoretical weight: 52.36 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q10103 (Residues: 504-960; Coverage: 48%)
Gene names: SPAC18G6.02c, chp1
Sequence domains: Chromo domain-containing protein 1-like, PIN domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 8 x CL
Ligand K 3 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 104.88Å b: 172.2Å c: 198.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.243
Expression system: Spodoptera frugiperda

Quick links

Citations

8 review citations

Different means, same end-heterochromatin formation by RNAi and RNAi-independent RNA processing factors in fission yeast.
Reyes-Turcu et al. (2012)
7 more

4 mentions without citation

Structural conservation of the PIN domain active site across all domains of life.
Senissar et al. (2017)
3 more