› 3tjk

X-ray diffraction
2.09Å resolution

Crystal Structure of human peroxiredoxin IV C245A mutant in reduced form

Released:
DOI: 10.2210/pdb3tjk/pdb
PDB entry 3tjk coloured by chain, front view.
PDB entry 3tjk coloured by chain, side view.
PDB entry 3tjk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of reduced and of oxidized peroxiredoxin IV enzyme reveals a stable oxidized decamer and a non-disulfide-bonded intermediate in the catalytic cycle.
Cao Z, Tavender TJ, Roszak AW, Cogdell RJ, Bulleid NJ
J Biol Chem 286 42257-42266 (2011)
PMID: 21994946

Function and Biology Details

Reaction catalysed: 
Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171577 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxiredoxin-4 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 254 amino acids
Theoretical weight: 28.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13162 (Residues: 38-271; Coverage: 100%)
Gene name: PRDX4
Sequence domains:
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 108.24Å b: 138.4Å c: 96Å
α: 90° β: 103.8° γ: 90°
R-values:
R R work R free
0.162 0.16 0.201
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 review citations

Peroxiredoxins: guardians against oxidative stress and modulators of peroxide signaling.
Perkins et al. (2015)
24 more

3 mentions without citation

Peroxiredoxins wear many hats: Factors that fashion their peroxide sensing personalities.
Bolduc et al. (2021)
2 more