› 3tm6

X-ray diffraction
2.7Å resolution

Crystal structure of the beta-2 microglobulin DIMC50 disulphide-linked homodimer mutant

Released:
DOI: 10.2210/pdb3tm6/pdb
PDB entry 3tm6 coloured by chain, front view.
PDB entry 3tm6 coloured by chain, side view.
PDB entry 3tm6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A recurrent D-strand association interface is observed in β-2 microglobulin oligomers.
Colombo M, de Rosa M, Bellotti V, Ricagno S, Bolognesi M
FEBS J 279 1131-43 (2012)
PMID: 22289140

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158378 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-2-microglobulin Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 100 amino acids
Theoretical weight: 11.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand PEG 5 x PEG
Ligand PO4 6 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 90.102Å b: 56.272Å c: 96.374Å
α: 90° β: 115.67° γ: 90°
R-values:
R R work R free
0.235 0.234 0.262
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structural, morphological, and functional diversity of amyloid oligomers.
Breydo et al. (2015)
11 other articles

3 mentions without citation

The Early Phase of β2-Microglobulin Aggregation: Perspectives From Molecular Simulations.
Loureiro et al. (2020)
2 more