› 3tpm

X-ray diffraction
2.1Å resolution

Crystal structure of MAL RPEL domain in complex with importin-alpha

Released:
DOI: 10.2210/pdb3tpm/pdb
PDB entry 3tpm coloured by chain, front view.
PDB entry 3tpm coloured by chain, side view.
PDB entry 3tpm coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Sensing actin dynamics: structural basis for G-actin-sensitive nuclear import of MAL.
Hirano H, Matsuura Y
Biochem Biophys Res Commun 414 373-8 (2011)
PMID: 21964294

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156505 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 422 amino acids
Theoretical weight: 45.8 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 75-496; Coverage: 80%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
MAL Chain: B
Molecule details ›
Chain: B
Length: 120 amino acids
Theoretical weight: 14.06 KDa
Source organism: Mus musculus
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 78.658Å b: 90.116Å c: 98.853Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.208
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Modular peptide binding: from a comparison of natural binders to designed armadillo repeat proteins.
Reichen et al. (2014)
3 more

4 mentions without citation

Design rules for selective binding of nuclear localization signals to minor site of importin α.
Pang et al. (2014)
3 more