› 3tq7

X-ray diffraction
2.3Å resolution

EB1c/EB3c heterodimer in complex with the CAP-Gly domain of P150glued

Released:
DOI: 10.2210/pdb3tq7/pdb
PDB entry 3tq7 coloured by chain, front view.
PDB entry 3tq7 coloured by chain, side view.
PDB entry 3tq7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Interaction of mammalian end binding proteins with CAP-Gly domains of CLIP-170 and p150(glued).
Bjelić S, De Groot CO, Schärer MA, Jaussi R, Bargsten K, Salzmann M, Frey D, Capitani G, Kammerer RA, Steinmetz MO
J Struct Biol 177 160-7 (2012)
PMID: 22119847

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-171968 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Microtubule-associated protein RP/EB family member 1 Chain: A
Molecule details ›
Chain: A
Length: 78 amino acids
Theoretical weight: 9.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15691 (Residues: 191-268; Coverage: 29%)
Gene name: MAPRE1
Sequence domains: EB1-like C-terminal motif
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Microtubule-associated protein RP/EB family member 3 Chain: B
Molecule details ›
Chain: B
Length: 82 amino acids
Theoretical weight: 9.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UPY8 (Residues: 200-281; Coverage: 29%)
Gene name: MAPRE3
Sequence domains: EB1-like C-terminal motif
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Dynactin subunit 1 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 71 amino acids
Theoretical weight: 7.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14203 (Residues: 27-97; Coverage: 6%)
Gene name: DCTN1
Sequence domains: CAP-Gly domain
Structure domains: CAP Gly-rich-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 41.15Å b: 103.56Å c: 48.13Å
α: 90° β: 113.64° γ: 90°
R-values:
R R work R free
0.229 0.226 0.281
Expression system: Escherichia coli

Quick links

Citations

4 review citations

+TIPs: SxIPping along microtubule ends.
Kumar et al. (2012)
3 more

2 mentions without citation

Mechanism and regulation of cytoplasmic dynein.
Cianfrocco et al. (2015)
1 more