› 3trt

X-ray diffraction
2.3Å resolution

Crystal structure of stabilised vimentin coil2 fragment

Released:
DOI: 10.2210/pdb3trt/pdb
PDB entry 3trt coloured by chain, front view.
PDB entry 3trt coloured by chain, side view.
PDB entry 3trt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Stabilization of vimentin coil2 fragment via an engineered disulfide.
Chernyatina AA, Strelkov SV
J Struct Biol 177 46-53 (2012)
PMID: 22119849

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140284 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vimentin Chains: A, B
Molecule details ›
Chains: A, B
Length: 77 amino acids
Theoretical weight: 8.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08670 (Residues: 261-335; Coverage: 16%)
Gene name: VIM

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 3 x GOL
Ligand NH4 1 x NH4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: I222
Unit cell:
a: 53.419Å b: 75.521Å c: 86.859Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.225 0.298
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Intermediate Filaments: Structure and Assembly.
Herrmann et al. (2016)
2 more

13 mentions without citation

Atomic structure of the vimentin central α-helical domain and its implications for intermediate filament assembly.
Chernyatina et al. (2012)
12 more