› 3trz

X-ray diffraction
2.9Å resolution

Mouse Lin28A in complex with let-7d microRNA pre-element

Released:
DOI: 10.2210/pdb3trz/pdb
PDB entry 3trz coloured by chain, front view.
PDB entry 3trz coloured by chain, side view.
PDB entry 3trz coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis for interaction of let-7 microRNAs with Lin28.
Nam Y, Chen C, Gregory RI, Chou JJ, Sliz P
Cell 147 1080-91 (2011)
PMID: 22078496

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185071 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein lin-28 homolog A Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 148 amino acids
Theoretical weight: 16.07 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8K3Y3 (Residues: 31-187; Coverage: 71%)
Gene names: Lin28, Lin28a, Tex17
Sequence domains:
Structure domains:
RNA (5'-R(*GP*GP*GP*CP*AP*GP*GP*GP*AP*UP*UP*UP*UP*GP*CP*CP*CP*GP*GP*AP*G)-3') Chains: U, V, W, X, Y, Z
Molecule details ›
Chains: U, V, W, X, Y, Z
Length: 21 nucleotides
Theoretical weight: 6.76 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 12 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P43212
Unit cell:
a: 143.82Å b: 143.82Å c: 177.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.21
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

63 review citations

Regulation of microRNA biogenesis.
Ha et al. (2014)
62 more

6 mentions without citation

Mechanisms of Lin28-mediated miRNA and mRNA regulation--a structural and functional perspective.
Mayr et al. (2013)
5 more