› 3ts0

X-ray diffraction
2.76Å resolution

Mouse Lin28A in complex with let-7f-1 microRNA pre-element

Released:
DOI: 10.2210/pdb3ts0/pdb
PDB entry 3ts0 coloured by chain, front view.
PDB entry 3ts0 coloured by chain, side view.
PDB entry 3ts0 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Molecular basis for interaction of let-7 microRNAs with Lin28.
Nam Y, Chen C, Gregory RI, Chou JJ, Sliz P
Cell 147 1080-91 (2011)
PMID: 22078496

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185072 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein lin-28 homolog A Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 15.92 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8K3Y3 (Residues: 33-187; Coverage: 70%)
Gene names: Lin28, Lin28a, Tex17
Sequence domains:
Structure domains:
RNA (5'-R(*GP*GP*GP*GP*UP*AP*GP*UP*GP*AP*UP*UP*UP*UP*AP*CP*CP*CP*UP*GP*GP*AP*G)-3') Chains: U, V
Molecule details ›
Chains: U, V
Length: 23 nucleotides
Theoretical weight: 7.36 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P41212
Unit cell:
a: 139.88Å b: 139.88Å c: 85.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.214
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

63 review citations

Regulation of microRNA biogenesis.
Ha et al. (2014)
62 more

9 mentions without citation

Mechanisms of Lin28-mediated miRNA and mRNA regulation--a structural and functional perspective.
Mayr et al. (2013)
8 more