› 3ts4

X-ray diffraction
1.59Å resolution

Human MMP12 in complex with L-glutamate motif inhibitor

Released:
DOI: 10.2210/pdb3ts4/pdb
PDB entry 3ts4 coloured by chain, front view.
PDB entry 3ts4 coloured by chain, side view.
PDB entry 3ts4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Simple pseudo-dipeptides with a P2' glutamate: a novel inhibitor family of matrix metalloproteases and other metzincins.
Devel L, Beau F, Amoura M, Vera L, Cassar-Lajeunesse E, Garcia S, Czarny B, Stura EA, Dive V
J Biol Chem 287 26647-56 (2012)
PMID: 22689580

Function and Biology Details

Reaction catalysed: 
Hydrolysis of soluble and insoluble elastin (1). Specific cleavages are also produced at -Ala(14)-|-Leu- and -Tyr(16)-|-Leu- in the B chain of insulin (2).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153971 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage metalloelastase Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 17.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P39900 (Residues: 106-263; Coverage: 35%)
Gene names: HME, MMP12
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

5 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 3 x CA
Ligand EEG 1 x EEG
Ligand IMD 1 x IMD
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 70.09Å b: 63.65Å c: 37.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.203
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Matrix metalloproteinases as breast cancer drivers and therapeutic targets.
Radisky et al. (2015)
5 more

2 mentions without citation

Simple pseudo-dipeptides with a P2' glutamate: a novel inhibitor family of matrix metalloproteases and other metzincins.
Devel et al. (2012)
1 more