› 3tsw

X-ray diffraction
2.85Å resolution

crystal structure of the PDZ3-SH3-GUK core module of Human ZO-1

Released:
DOI: 10.2210/pdb3tsw/pdb
PDB entry 3tsw coloured by chain, front view.
PDB entry 3tsw coloured by chain, side view.
PDB entry 3tsw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Src homology 3 domain is required for junctional adhesion molecule binding to the third PDZ domain of the scaffolding protein ZO-1.
Nomme J, Fanning AS, Caffrey M, Lye MF, Anderson JM, Lavie A
J Biol Chem 286 43352-60 (2011)
PMID: 22030391

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-170447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tight junction protein ZO-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 391 amino acids
Theoretical weight: 44.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q07157 (Residues: 417-803; Coverage: 22%)
Gene names: TJP1, ZO1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P32
Unit cell:
a: 100.634Å b: 100.634Å c: 182.516Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.211 0.29
Expression system: Escherichia coli BL21

Quick links

Citations

18 review citations

Structures and target recognition modes of PDZ domains: recurring themes and emerging pictures.
Ye et al. (2013)
17 more

2 mentions without citation

The Src homology 3 domain is required for junctional adhesion molecule binding to the third PDZ domain of the scaffolding protein ZO-1.
Nomme et al. (2011)
1 more