› 3tsz

X-ray diffraction
2.5Å resolution

crystal structure of PDZ3-SH3-GUK core module from human ZO-1 in complex with 12mer peptide from human JAM-A cytoplasmic tail

Released:
DOI: 10.2210/pdb3tsz/pdb
PDB entry 3tsz coloured by chain, front view.
PDB entry 3tsz coloured by chain, side view.
PDB entry 3tsz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Src homology 3 domain is required for junctional adhesion molecule binding to the third PDZ domain of the scaffolding protein ZO-1.
Nomme J, Fanning AS, Caffrey M, Lye MF, Anderson JM, Lavie A
J Biol Chem 286 43352-60 (2011)
PMID: 22030391

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170448 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tight junction protein ZO-1 Chain: A
Molecule details ›
Chain: A
Length: 391 amino acids
Theoretical weight: 44.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q07157 (Residues: 417-803; Coverage: 22%)
Gene names: TJP1, ZO1
Sequence domains:
Structure domains:
Junctional adhesion molecule A Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.37 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9Y624 (Residues: 288-299; Coverage: 4%)
Gene names: F11R, JAM1, JCAM, UNQ264/PRO301

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 50.5Å b: 49.7Å c: 91.8Å
α: 90° β: 101.1° γ: 90°
R-values:
R R work R free
0.247 0.24 0.309
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

18 review citations

Structures and target recognition modes of PDZ domains: recurring themes and emerging pictures.
Ye et al. (2013)
17 more

5 mentions without citation

The Src homology 3 domain is required for junctional adhesion molecule binding to the third PDZ domain of the scaffolding protein ZO-1.
Nomme et al. (2011)
4 more