› 3tw4

X-ray diffraction
3.35Å resolution

Crystal Structure of Human Septin 7 GTPase Domain

Released:
DOI: 10.2210/pdb3tw4/pdb
PDB entry 3tw4 coloured by chain, front view.
PDB entry 3tw4 coloured by chain, side view.
PDB entry 3tw4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Promiscuous interactions of human septins: the GTP binding domain of SEPT7 forms filaments within the crystal.
Serrão VH, Alessandro F, Caldas VE, Marçal RL, Pereira HD, Thiemann OH, Garratt RC
FEBS Lett 585 3868-73 (2011)
PMID: 22064074

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172448 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Septin-7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 271 amino acids
Theoretical weight: 31.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16181 (Residues: 48-318; Coverage: 62%)
Gene names: CDC10, SEPT7, SEPTIN7
Sequence domains: Septin
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P61
Unit cell:
a: 82.51Å b: 82.51Å c: 210.91Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.293 0.289 0.365
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The Structural Biology of Septins and Their Filaments: An Update.
Cavini et al. (2021)
10 other articles

4 mentions without citation

Septin structure and filament assembly.
Valadares et al. (2017)
3 more