› 3twq

X-ray diffraction
2.15Å resolution

Crystal structure of ARC4 from human Tankyrase 2 (apo form)

Released:
DOI: 10.2210/pdb3twq/pdb
PDB entry 3twq coloured by chain, front view.
PDB entry 3twq coloured by chain, side view.
PDB entry 3twq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis and sequence rules for substrate recognition by Tankyrase explain the basis for cherubism disease.
Guettler S, LaRose J, Petsalaki E, Gish G, Scotter A, Pawson T, Rottapel R, Sicheri F
Cell 147 1340-54 (2011)
PMID: 22153077

Function and Biology Details

Reaction catalysed: 
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190552 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly [ADP-ribose] polymerase tankyrase-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 175 amino acids
Theoretical weight: 18.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H2K2 (Residues: 484-655; Coverage: 15%)
Gene names: PARP5B, TANK2, TNKL, TNKS2
Sequence domains: Ankyrin repeats (3 copies)
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P3112
Unit cell:
a: 47.524Å b: 47.524Å c: 250.935Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.174 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

47 review citations

Classification of intrinsically disordered regions and proteins.
van der Lee et al. (2014)
46 more

2 mentions without citation

Macrocyclized Extended Peptides: Inhibiting the Substrate-Recognition Domain of Tankyrase.
Xu et al. (2017)
1 more